Since the role of CARM1 in estrogen-dependent breast cancer was confirmed (62), PRMT6-siRNA-1 assay showed that PRMT6 also played a role in estrogen signaling, and the survival rate of MCF-7 cells was significantly reduced, participating in estrogen-stimulated ERα-expressing breast cancer cell proliferation (13). This evidence concerns the gene ESR1 and breast carcinoma.