It was observed that the expression of gene encoding PRMT6 was increased in rhabdomyosarcoma cell lines, and PRMT inhibitors (AMI-1 and SAH) effectively reduced the invasive phenotype of RMS cells by inhibiting the proliferation rate, cell viability and colony formation ability of rhabdomyosarcoma cell lines. This evidence concerns the gene PRMT6 and rhabdomyosarcoma.