Furthermore, we found that the average combined NE score of tumors in either POU2F3 (median = 88) or YAP1 (median = 63) subtypes were dramatically lower than tumor in either ASCL1 (median = 165) or NEUROD1 (median = 156) subtypes, which was consistent with previous report (12, 15) and suggested that the degree of NE differentiation may contribute to the evolution of molecular subtypes. This evidence concerns the gene ASCL1 and neoplasm.