The expression of TRPM5, TRPM3, PLCB4, ITPR1, PTGER2, ADCY6, PRKACB, and PRKCG was obviously declined in ESCC tissues by comparing with that in normal tissues, whereas TRPV4, TRPV3, HTR2C, PLA2G4E, ASIC5, CALML3, CALML5, IL1RAP, MAPK12, and NGF expression were significantly increased. The gene discussed is ADCY6; the disease is esophageal squamous cell carcinoma.