As such, we investigated the link between tumor mutations and the GTMEIscore and found that compared to the low GTMEIscore group, the GTMEIscore group had significantly higher PTEN and NF1 mutation rates (Figure 3E and Supplementary Figure 2C), and PTEN and NF1 mutations have been shown to cause increased infiltration of TAMs into tumor tissue (26, 27, 35). The gene discussed is PTEN; the disease is neoplasm.