We then analyzed the differences in the distribution of somatic mutations between the high and low GTMEIscore groups in the TCGA-GBM cohort and Wang cohort using the “maftools” package and found that the PTEN mutation rate (low: 22%, high: 35%) was significantly increased in the high GTMEIscore group compared to the low GTMEIscore group (Figure 3F and Supplementary Figure 2E). This evidence concerns the gene PTEN and glioblastoma.