Recently, it has been shown that a single 10 Gy dose of radiation can induce an increase in tumor associated macrophages in SHH medulloblastoma (33), however, in our study using a clinically-relevant fractionated CSI protocol did not result in an increase in absolute counts of microglia nor macrophage at either acute or late-stage time points, and no increase in Iba1+ staining was observed in late-stage tumors after CSI (Supplementary Figure 8). This evidence concerns the gene AIF1 and medulloblastoma.