To determine the effect of the MAP3K1R186G variant on kinase and ovarian-specific pathways, we evaluated phosphorylation levels of p38 and GSK3β as well as activation of the Wnt4/β-catenin pathway in the testicular teratoma cells (NT2/D1) and ovary-derived granular cells (KGN). This evidence concerns the gene GSK3B and testicular teratoma.