To the best of our knowledge, previous studies usually measured the cytokines over weeks or months after IV-anti-VEGF treatment, and mainly for diabetic macular edema (DME) (Jeon and Lee, 2012; Hillier et al., 2018; Mastropasqua et al., 2018; Cacciamani et al., 2019; Felfeli et al., 2019; Wei et al., 2019; Juncal et al., 2020; Imazeki et al., 2021; Xavier et al., 2021), few focused on the early-stage changes of cytokines after IV-anti-VEGF. The gene discussed is VEGFA; the disease is diabetic macular edema.