It has been found that the RRP replenishment is suppressed by a variety of manipulations that inhibit F-actin, including knockout of the β-actin or γ-actin gene (Figures 2A,B), Lat A that inhibits F-actin polymerization (Figure 2C), Kv3.3 that disrupts F-actin nucleation, and mutation of Kv3.3 that causes spinocerebellar ataxia and inhibits F-actin nucleation at nerve terminals (Sakaba and Neher, 2003; Wu et al., 2016, 2021). Here, KCNC3 is linked to cerebellar ataxia.