In our study, using the combination of an integrated proteomics and metabolomics strategy and IPA bioinformatic analysis, we screened the metabolic profiling, and protein profiling was significantly changed in CaOx crystal-induced kidney injury mice and found that the CaOx crystal could induce inflammatory reactions and oxidative stress through Akt, ERK1/2, p38 MAPK pathways and affect amino acid metabolism and fatty acid β-oxidation, resulting in renal injury. This evidence concerns the gene AKT1 and kidney injury.