To further investigate the connection between HSPA6 and these non-EV71 HFMD-associated viruses, the cells were transfected with 100 nM of si-HSPA6 or si-NC for 24 h and then infected with the viruses for 72 h, followed by the cell survival detection with Alamar blue reagent. Here, HSPA6 is linked to hand, foot and mouth disease.