Firstly, as the initiating factor of the inflammatory cascade, TNF-α regulates downstream NF-κB and MAPK signaling pathways through its receptor to promote the release of IL-6, IL-8, IL-1, and other inflammatory factors by macrophages, thereby inducing the activation of macrophages, lymphocytes, and other cells, and aggravating vascular endothelial injury and deep venous thrombosis (27). This evidence concerns the gene CXCL8 and Venous thrombosis.