The RT–PCR results showed that the mRNA expression of NLRP1, TNFα, CASP1, CASP9, and PRKACA in DCM myocardial tissues was significantly higher than that in normal myocardial tissues (P < 0.05), consistent with the results of bioinformatics analysis (Figure 8(c). The gene discussed is TNF; the disease is familial dilated cardiomyopathy.