Five candidate genes associated with PKLR were validated by a Pearson correlation analysis, and we found that ACSL3, APPBP2, PIAS1, and ZBTB10 were significantly negatively correlated with PKLR based on significant p values (of <0.0001) in the two PCa datasets (Supplementary Fig. S2E). The gene discussed is PIAS1; the disease is posterior cortical atrophy.