Then, we would like to determine whether the loss function of circ_0001006 performs inhibition function on cardiac hypertrophy, and we, therefore, created short hairpin RNA (shRNA) sh-circ_0001006 or sh-Scramble (sh-Scr) and transfected into cardiomyocytes treated with Ang II (200 nmol/L) for 48 hrs (Figure 4A). This evidence concerns the gene AGT and cardiac hypertrophy.