Due to the high expression level of RSPH14 in HCC tissues and its relationship with poor clinical outcome, we constructed the lentivirus containing shRNA for targeting RSPH14 and transfected BEL-7404 and SMMC-7721 cells with the lentiviral vectors to downregulate RSPH14 expression to investigate the role of RSPH14 in HCC. The gene discussed is RSPH14; the disease is hepatocellular carcinoma.