To study the effect of METTL1 on ESCC progression in vivo, we first induced ESCC formation in the Mettl1 cKO and control mice, then the tumor-bearing mice were treated with tamoxifen to induce Mettl1 knockout in the cKO mice (Supplementary Fig. 11a). The gene discussed is METTL1; the disease is esophageal squamous cell carcinoma.