Similarly, inhibition of WDR4 led to decreased m7G modification level and m7G-modified tRNA expression; and overexpression of WDR4 increased m7G tRNA modification and m7G-modified tRNA expression in both K150 and K30 ESCC cells (Fig. 3i, j and Supplementary Fig. 4c, d). Here, WDR4 is linked to esophageal squamous cell carcinoma.