To explore the potential mechanisms underlying METTL1/WDR4 overexpression in ESCCs, we first analyzed the METTL1 and WDR4 genome and found that small percentage of ESCC patients contain DNA amplification, mutation, or deletion on METTL1 or WDR4 genes (Supplementary Fig. 1e). Here, METTL1 is linked to esophageal squamous cell carcinoma.