To elucidate whether the elevated S100a9 signaling in cancers could increase MDSC accumulation in mice, we generated doxycycline (DOX) S100a9-inducible B477 cells (Brca1-WT-Dox) and G600 cells (Brca1-MT-Dox) (Supplementary Fig. 6d, e). Here, BRCA1 is linked to cancer.