Western blotting, immunofluorescence, mass spectrometry, and rescue assay demonstrated the involvement of NF-κB in the UBAP2L-induced EMT process in GC cells and elucidated that the activation of NF-κB is dependent on the activation of the PI3K/AKT/ SP1 axis (Fig. 7). This evidence concerns the gene AKT1 and gastric cancer.