Low-dose EMPA (100 nM) effects in the presence of albumin on hyperglycaemia-induced senescence of native and freshly isolated porcine coronary artery endothelial cells, show that EMPA reduced high glucose-induced ROS production and restored eNOS expression and NO formation through prevention of the activation of the endogenous angiotensin (Ang) system [39]. This evidence concerns the gene ANG and Hyperglycemia.