MAP1LC3B and Langer mesomelic dysplasia: The role of the stable hairpin structure in LMD was further validated through in vivo CLIP experiments using α-LC3B antibody and qRT-PCR using RLuc-P3′-WT mRNA, RLuc-P3′-M3 mRNA (which harbors seven nucleotide substitutions in the stem region and consequently disrupts the hairpin structure), or RLuc-P3′-M4 mRNA (which harbors an additional seven nucleotide substitutions in the complementary strand in the M3 backbone and consequently restores the stable hairpin structure) (Fig. 4c).