CTLA4 and neoplasm: Manual gating (Supplementary Fig. 4a) validated that PD-1+GB−CD8+ exhausted T cells, Foxp3+CD152+CD4+ Treg, and PD-1+CD45RO+CD4+ memory T cells were enriched; while GB+CD56+ active NK cells and PD-1−GB+CD8+ activated T cells were depleted, specifically in S2 tumours compared to S1 or S3 tumours or both (Fig. 2e).