To determine whether neuropathy-causing mutations might impact ubiquitination of the TRPV4 N-terminal IDR, we performed cell-based Ub assays examining transiently transfected HEK293T cells, as well as HEK293T cell lines that inducibly express either WT (T-Rex-TRPV4WT) or R269C neuropathy mutant (T-Rex-TRPV4R269C) FLAG-tagged TRPV4 (32). This evidence concerns the gene TRPV4 and neuropathy.