The data suggested that treatment with CM of activated macrophages enhanced migration of 4T1 cells by 2.2-fold as compared to control which was significantly suppressed by IL-6 neutralization (1.3-fold) or Stattic treatment (1.1-fold) indicating that TAM derived IL-6 promotes breast cancer cell migration via STAT-3 pathway (Fig. 5A). This evidence concerns the gene IL6 and breast cancer.