We examined the levels of p-STAT3 expression in response to CM of TAMs and/or recombinant IL-6 in breast cancer cells and our results exhibited enhanced phosphorylation of STAT-3 upon treatment with either recombinant IL-6 or CM of activated macrophages and the effect was abrogated when IL-6 was neutralized in CM of activated RAW264.7 cells suggesting that IL-6 may act through JAK/STAT pathway to regulate CSCs in breast cancer. The gene discussed is STAT3; the disease is breast carcinoma.