Figure 1 shows the distribution of E-cadherin (green) increases as the tumor breeches a smooth muscle barrier (white dotted line). Tumor clusters that are moving between the muscle layers (Figure 1A) express E-cadherin within the central region of the cluster, with α6 integrin observed in a distinct cell-ECM distribution (Figure 1B) with no detectable overlap with E-cadherin. This observation is consistent with previous work that aggressive prostate tumors within metastatic bone sites express E-cadherin (De Marzo et al., 1999). This evidence concerns the gene CDH1 and neoplasm.