And we further detected the expression of ASCL2 and LGR5, two main Wnt target genes in CRC, in above 298 cases of CRC patients, and found that expression of RAI2 was negatively correlated with that of ASCL2 (r=-0.1674, P=0.0037) and LGR5 (r=-0.1580, P=0.0063) (Figure 4A). The gene discussed is ASCL2; the disease is colorectal carcinoma.