Low expression of RAI2 was found in 33.89% (101/298) of the colorectal cancer samples, and the expression of RAI2 was positively correlated with phosphorylated β-catenin (r=0.8866, P<0.0001), which is further validation of the inhibitory effect of RAI2 on Wnt/β-catenin signaling. This evidence concerns the gene RAI2 and colorectal cancer.