It is interesting to notice, that enriched gene sets associated with database gene sets GO:0007173 (epidermal growth factor receptor signaling pathway) and ko04012 (ErbB signaling pathway) were differently regulated after E2 treatment, since intracellular signaling pathways of ErbB and other epidermal growth factor family members have been identified to control proliferation, differentiation and cell cycle progress and are valuable targets in cancer targeted therapy [26, 27]. Here, EGFR is linked to cancer.