To determine whether a correlation between O-GlcNAcylation and PI-3 kinase/Akt pathway could be observed in human cervical cancer, we evaluated Akt phosphorylation (P-Akt) and protein O-GlcNAcylation levels in cervical cancer tissues and healthy cervix by immunofluorescence staining. The gene discussed is AKT1; the disease is cervical carcinoma.