Moreover, GNE-493 (250 nM)-induced death (Fig. 3G) and apoptosis (Fig. 3H) in primary prostate cancer cells were more potent than the same concentration of LY294002 (the pan PI3K-Akt-mTOR inhibitor [19]) or INK-128 (the mTOR kinase inhibitor [13]) in priCa-1 or priCa-2 primary cancer cells. This evidence concerns the gene AKT1 and Familial prostate cancer.