In priCa-1 primary cancer cells, GNE-493 treatment (250 nM, 4 h) blocked phosphorylations of S6K1 (at the Thr-389 residue) and Akt (at the Ser-473 residue), confirming Akt-mTOR cascade blockage (Fig. 3A). This evidence concerns the gene AKT1 and cancer.