LAG3 and B-cell chronic lymphocytic leukemia: In Tregs A, we observed increased expression of genes responsible for immunosuppressive activity (Gzmb, Prf1, Gzmk, Il10), checkpoints (Havcr 2, Lag-3, Tigit), chemokines that may support leukemia progression and its microenvironment (Ccl3, Csf1, Ccl5), as well as genes that have been recently reported as unique for CLL-Tregs (EOMES) (Figure 3D).