CD44 and leukemia: Based on the results of phenotyping with a set of markers (FoxP3, LAG-3, CD69, CD44, CD25), we performed t-distributed stochastic neighbor embedding (tSNE) analysis, which allowed us to distinguish a specific Tregs subpopulation that exerts the phenotype characteristic only for Tregs isolated from TCL1 leukemia-bearing mice (Tregs A) (Figure 3A).