CASP3 and Miyoshi myopathy: Q-VD-OPH, a pan-caspase suppressor, forms covalent bonds with and permanently sequesters caspase-3 (18).So we detected the GSDME- N and GSDME- F protein expressions in two cell lines incubated with DOX and Q-VD-OPH alone or in combination western blot analysis (Figure 7F) to confirm that DOX-induced pyroptosis is drastically reduced by Q-VD-OPH in MM cells.