The pan-Trk staining showed three different cellular distribution patterns, specifically: isolated cytoplasmic staining was the predominate pattern found, as it occurred in 9/12 cases (7 Ewing sarcoma, 1 USTS and 1 sarcoma with BCOR genetic alteration) while a nuclear staining in 2/12 cases (2 USTS) and a membranous staining in 1/12 cases (1 Ewing sarcoma). This evidence concerns the gene TPM3 and Ewing sarcoma.