A total of five variables, including patient age (p < 0.001), tumor location (p = 0.017), differentiation type (p < 0.001), CEA level (p = 0.038) and the presence of PNI (p < 0.001), were shown to be associated with increased risk of lymph node metastasis according to the results of univariate analysis. This evidence concerns the gene CEACAM5 and metastatic malignant neoplasm in the lymph nodes.