A total of five variables, including patient age (p < 0.001), tumor location (p = 0.017), differentiation type (p < 0.001), CEA level (p = 0.038) and the presence of PNI (p < 0.001), were shown to be associated with increased risk of lymph node metastasis according to the results of univariate analysis. This evidence concerns the gene CEACAM5 and neoplasm.