The results showed that age was older (p < 0.001), the proportions of rectum (p = 0.017), elevated CEA level (p = 0.038) as well as poorly differentiation/MUC/SRC (p < 0.001) were higher, and PNI (p < 0.001) was more frequent in patients with lymph node metastasis than those without lymph node metastasis (Table1). The gene discussed is CEACAM5; the disease is metastatic malignant neoplasm in the lymph nodes.