We have previously demonstrated that in the rat RM-AKI model—which is the same model used in this study—following glycerol injection, there was a significant increase in endogenous HO-1 expression and a rapid and robust decrease in ALAS1 expression, suggesting a significant improvement in the renal intracellular free-heme concentration after glycerol treatment [11]. The gene discussed is HMOX1; the disease is acute kidney injury.