We proposed that there was a marked rise in intracellular heme concentration in the renal system of RM-AKI rats based on dynamic changes in three substances, including HO-1, δ-aminolevulinate synthase (ALAS1)—a key enzyme involved in heme metabolism that is usually downregulated by heme—and the nuclear Broad-complex, Tramtrack and Bric-a-brac (BTB), and cap’n’collar (CNC) homology proteins 1 (Bach1), a heme responsive transcription factor, which is exported from the nucleus and binds to intracellular heme. Here, HMOX1 is linked to acute kidney injury.