To sum up, this study uncovered the impacts of LEMD1 on the malignant properties of colon cancer cells and identified that LEMD1 transcriptionally activated by SOX4 stimulated the PI3K/AKT signaling, which for the first time revealed the mechanism by which LEMD1 facilitated the development of colon cancer. This evidence concerns the gene SOX4 and malignant colon neoplasm.