Meanwhile, transfection of NLS-YBX1 into GSDME−/− AsPC-1 and PANC-1 cells rescued the resistance to trypsin and chymotrypsin (Fig. 5f and Extended Data Fig. 5j); however, the transfection of NLS-GSDME into YBX1−/− tumour cells did not have such an effect (Extended Data Fig. 5k). This evidence concerns the gene YBX1 and neoplasm.