These results seemed in accordance with violacein neuro-immunomodulatory and anti-inflammatory properties22,30, including the reduction of systemic levels of the inflammatory cytokines TNF-α, IL-1β and IL6, as well as the inhibition of MMP-2 and -930,31 that are elevated in limb muscles and spinal cord from hSOD1G93A ALS models and patients58, this protecting motor neurons and modulating inflammation32,33. This evidence concerns the gene MMP2 and amyotrophic lateral sclerosis.