On the other hand, the effect of TNFSF14 was also confirmed through transfection of the tnfsf14 gene into HCC cells, which showed that transfection with pcDNA3.1-tnfsf14 results in apparent increase of apoptosis in HepG2 and PLC/PRF/5 cells with different treatments (Fig. S3). Here, TNFSF14 is linked to hepatocellular carcinoma.