Additionally, we show that the overexpressed GFP-PTBP1 restored the impaired tumorigenesis and metastatic ability by depletion of PPP1R26 in mouse xenografts, further confirming that PPP1R26 may promote the tumorigenesis and metastasis in HCC via collaborating with PTBP1 in vivo. Here, PTBP1 is linked to hepatocellular carcinoma.