To further investigate the cross-talk between the GSK3α and HIF1/VEGFA signaling pathways, we stably expressed vector + shnc, GSK3α + shnc, GSK3α + shHIF1α#1, and GSK3α + shHIF1α#2 in NSCLC cell lines, then performed western blot and ELISA assays to analyze protein expression. The gene discussed is HIF1A; the disease is non-small cell lung carcinoma.