We also detected the expression of ki67, phosphorylated AKT (p-AKT), p-mTOR, and p-CHEK1, which are closely related to p53, cell proliferation, and growth, by immunohistochemistry and found the high expression of ki67, mutant p53, p-CHEK1, p-AKT, and p-mTOR in colorectal tissues in the AOM/DSS group that was treated with 0.9% NaCl. This evidence concerns the gene CHEK1 and infectious otitis media.