Considering the consistency of the network pharmacology prediction and RNA-Seq verification results of SW620 cells and considering that SW620 cells are more representative of CRC than SW480 cells, we selected p53, CHEK1, CCND1, CDKN2A, MDM2, and p21 as key targets of CKI-induced cell cycle arrest. The gene discussed is CHKA; the disease is colorectal carcinoma.