As mentioned above, preliminary verification by RNA-Seq at the cell level showed that the cell cycle, p53 signaling pathway, FoxO signaling pathway, ErbB signaling pathway, Viral carcinogenesis, and proteoglycans in cancer were key signaling pathways that are targeted by CKI, and most of them are involved in cell cycle progression, proliferation, apoptosis, autophagy, and growth of CRC cells. The gene discussed is CHKA; the disease is cancer.