In the present study, we found that CKI effectively inhibited the mRNA and protein expression of mutant p53 in SW480 and SW620 CRC cells in vitro (Fig. 8A–D) and inhibited p53 protein expression in colon tissue in the AOM/DSS-induced CRC model in vivo (Fig. 8E). The gene discussed is TP53; the disease is infectious otitis media.