Aggravated glucose intolerance of HFD-fed TfebΔβ-cell mice was accompanied by reduced β-cell function compared to HFD-fed TfebF/F mice as shown by decreased insulinogenic index (Fig. 6b) without significant changes of β-cell mass (Supplementary Fig. 10b), suggesting that Tfeb-KO β-cells could not functionally adapt to increased demand for insulin due to insufficient mitophagy and mitochondrial activity, leading to deteriorated glucose intolerance when metabolic stress was imposed. This evidence concerns the gene TFEB and Glucose intolerance.