To determine the type of genetic variation in DEMs of SLC25, such as DNA mutation, RNA alternative splicing and CNV, we utilized cBioProtal and found that mutations in SLC25A4, SLC25A5, SLC25A24, SLC25A34, SLC25A3, SLC25A51, SLC25A44, SLC25A12, SLC25A32, and SLC25A15 occurred at a higher frequency in patients with colon cancer than in healthy individuals, and the rates were 5, 6, 6, 10, 11, 11, 12, 16, 21, and 25, respectively (Fig. 3A). The gene discussed is SLC25A4; the disease is malignant colon neoplasm.