The combined effects of sorafenib and amiloride on tumor growth, the area of necrosis, and the levels of phosphorylated AKT, PAK1, and 4-HNE after blocking macropinocytosis were further verified in orthotopic RIL-175 tumors transplanted into C57BL/6 mice (Fig. 5F, G and Fig. S5H, I and J). Here, PAK1 is linked to neoplasm.