AKT1 and hepatocellular carcinoma: Consistent with the in vitro results, we found that combined treatment with amiloride attenuated sorafenib-induced dextran uptake and staining of phosphorylated AKT and PAK1 in HCC tissues from SK-Hep1 or Huh7 xenograft tumors (Fig. 5E and Fig. S5G), which highlights the important role of inhibiting macropinocytosis in increasing the susceptibility of HCC to sorafenib.