Our data showed the upregulation of SIX1 in DDP-resistant NSCLC samples and cell lines, and SIX1 overexpression lessened the repressive effect of miR-1296-5p overexpression on DDP resistance in DDP-resistant NSCLC cells, manifesting that miR-1296-5p could regulate DDP resistance via targeting SIX1 in DDP-resistant NSCLC cells. The gene discussed is SIX1; the disease is non-small cell lung carcinoma.