In multivariable linear regression models adjusting for age, race-ethnicity and BMI, both estimated glomerular filtration rate (B = 2.21, 95% CI 1.41–3.01, p < 0.001) and hemoglobin A1c (B = 37.38, 95% CI 28.91–45.86, p < 0.001) were positively associated with s-Klotho, while no significant association was found with cardiovascular disease. Here, KL is linked to cardiovascular disorder.