Ewing sarcoma-associated transcript 1 (lncRNA EWSAT1), located on human chromosome 15q23, only high expression in normal human testis and kidney, was recently reported more and more frequently to have intimate association with many different cancers, such as Ewing carcinoma [9], lung cancer [10], nasopharyngeal carcinoma [11], osteosarcoma [12–14], cervical cancer [15], colorectal cancer [16, 17], ovarian cancer [18], glioma [19]. This evidence concerns the gene EWSAT1 and osteosarcoma.