LEMD1 and pachyonychia congenita: To investigate the roles of LEMD1 in PC metastasis, fresh cancer cells (MIA PaCa-2, 6 × 106/100 μL; PANC-1, 5 × 106/100 μL) were all carefully injected into the tail vein of mice under anesthesia (a total of 10 mice for each group).