To determine the effect of ANGPTL4 silencing on NSCLC and energy metabolism in vivo, nude mice were subcutaneously inoculated with 1 × 106 A549 cells, and when the implanted tumours grew to 50 mm3 in one dimension, the mice were randomized and treated intravenously with vehicle as a control, pshRNA‐control or pshRNA‐ANGPTL4. The gene discussed is ANGPTL4; the disease is neoplasm.