Previously, the genetic overexpression technique of intact or mutant transporters such as ABCB4, ABCB11, ATP8b1, in human embryonic kidney (HEK293T) cells, intestinal cancer cell lines (Caco-2 cells), or Madin–Darby canine kidney II (MDCKII) cells has been used to study progressive cholestasis with genetic mutations.[26], [27], [28], [29], [30], [31] However, such an approach has limitations in recapitulating the physiological transport machinery of human hepatocytes, making them less optimal for studies of liver pathophysiology and drug screening.32 This evidence concerns the gene ATP8B1 and cholestasis.