To further explore whether NR2F1-AS1 affected the proliferation of PC cells in vivo, PANC-1 and MIA PaCa-2 cells stably transfected with a lentiviral vector containing sh-NR2F1-AS1#1, sh-NR2F1-AS1#2, or sh-Control were inoculated into nude mice. The gene discussed is NR2F1; the disease is pachyonychia congenita.