Given the close genomic proximity of NR2F1-AS1 and NR2F1, we hypothesized that NR2F1-AS1 could exert its effects by modulating NR2F1. NR2F1 knockdown was achieved in PC cells using two siRNAs; knockdown was verified at the mRNA and protein levels (Supplementary Fig. S6A, B). Here, NR2F1 is linked to pachyonychia congenita.